Comparative analysis of bovine serum albumin adsorption onto octacalcium phosphate crystals prepared using different methods

Kaori Tsuchiya, Ryo Hamai, Susumu Sakai, Osamu Suzuki

研究成果: Article査読

1 被引用数 (Scopus)

抄録

This study compared bovine serum albumin (BSA) adsorption onto octacalcium phosphate (OCP) materials prepared from two wet preparations in the absence (w-OCP) and presence (c-OCP) of gelatin. Raman spectroscopy was used to analyze the BSA adsorption onto OCPs in a 150 mM Tris-HCl buffer containing 0.5 mM calcium and inorganic phosphate (Pi) ions at pH 7.4 and at 37°C. The degree of supersaturation of the supernatants after the adsorption was determined by measuring the ion composition. The results showed that BSA adsorption onto w-OCP was higher than that for c-OCP. The calcium ion concentration of the supernatant decreased for both w-OCP and c-OCP, whereas the Pi ion concentration increased, approaching OCP equilibria at different saturation levels. BSA adsorbed even onto c-OCP, which included a small amount of gelatin during c-OCP preparation. These results indicate that the biodegradability of w-OCP and c-OCP may be modulated through interactions with serum proteins.

本文言語English
ページ(範囲)883-891
ページ数9
ジャーナルDental materials journal
39
5
DOI
出版ステータスPublished - 2020

ASJC Scopus subject areas

  • セラミックおよび複合材料
  • 歯科学(全般)

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