Comment on "evidence that the properdp method is inadequate for protein persulfidation detection due to lack of specificity"

Éva Dóka, Elias S.J. Arnér, Edward E. Schmidt, Tobias P. Dick, Albert Van Der Vliet, Jing Yang, Réka Szatmári, Tamás Ditrói, John L. Wallace, Giuseppe Cirino, Kenneth Olson, Hozumi Motohashi, Jon M. Fukuto, Michael D. Pluth, Martin Feelisch, Takaaki Akaike, David A. Wink, Louis J. Ignarro, Péter Nagy

研究成果: Article査読

抄録

The recent report by Fan et al. alleged that the ProPerDP method is inadequate for the detection of protein per sulfidation. Upon careful evaluation of their work, we conclude that the claim made by Fan et al. is not supported by their data, rather founded in methodological shortcomings. It is understood that the ProPerDP method generates a mixture of cysteine-containing and non cysteine-containing peptides. Instead, Fan et al. suggested that the detection of non cysteine-containing peptides indicates nonspecific alkylation at noncysteine residues. However, if true, then such peptides would not be released by reduction and therefore not appear as products in the reported workflow. Moreover, the authors biological assessment of ProPerDP using Escherichia coli mutants was based on assumptions that have not been confirmed by other methods. We conclude that Fan et al. did not rigorously assess the method and that ProPerDP remains a reliable approach for analyses of protein per/polysulfidation.

本文言語English
論文番号eabe7006
ジャーナルScience Advances
7
17
DOI
出版ステータスPublished - 2021 4 21

ASJC Scopus subject areas

  • 一般

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