TY - JOUR
T1 - Combined antibody and DNA detection for early diagnosis of leptospirosis after a disaster
AU - Iwasaki, Hiroko
AU - Chagan-Yasutan, Haorile
AU - Leano, Prisca Susan A.
AU - Koizumi, Nobuo
AU - Nakajima, Chie
AU - Taurustiati, Delsi
AU - Hanan, Firmanto
AU - Lacuesta, Talitha Lea
AU - Ashino, Yugo
AU - Suzuki, Yasuhiko
AU - Gloriani, Nina G.
AU - Telan, Elizabeth Freda O.
AU - Hattori, Toshio
N1 - Funding Information:
The study was supported by a Special Project Grant from the International Research Institute of Disaster Science, Tohoku University, and a grant from the Ministry of Education, Culture, Sports, Science, and Technology, Japan, for the Joint Research Program of the Research Center for Zoonosis Control, Hokkaido University.
Publisher Copyright:
© 2016 Elsevier Inc.
PY - 2016/4/1
Y1 - 2016/4/1
N2 - Early diagnosis based on laboratory confirmation is essential for managing leptospirosis. This study investigated the effectiveness of a novel method of detecting leptospirosis that combines measurement of anti-Leptospira antibodies by the microscopic agglutination test (MAT), enzyme-linked immunosorbent assay (ELISA), and immunochromatographic test (ICT) and leptospiral DNA by loop-mediated isothermal amplification (LAMP) and real-time PCR in plasma and 2 types of urine pellets. Of 113 suspected cases, 68.1%, 76.1%, and 60.2% were positive by MAT, ELISA, and ICT, respectively. Real-time PCR using DNA purified from urine pellets collected by low-speed centrifugation yielded positive signals for patients in late acute as well as early phase who were positive by LAMP using plasma DNA or urine pellets. Among antibody-negative patients, 9.5% were positive by DNA detection. These findings indicate that the leptospirosis detection rate is increased by combining antibody and DNA detection, providing a new tool for timely diagnosis of infection.
AB - Early diagnosis based on laboratory confirmation is essential for managing leptospirosis. This study investigated the effectiveness of a novel method of detecting leptospirosis that combines measurement of anti-Leptospira antibodies by the microscopic agglutination test (MAT), enzyme-linked immunosorbent assay (ELISA), and immunochromatographic test (ICT) and leptospiral DNA by loop-mediated isothermal amplification (LAMP) and real-time PCR in plasma and 2 types of urine pellets. Of 113 suspected cases, 68.1%, 76.1%, and 60.2% were positive by MAT, ELISA, and ICT, respectively. Real-time PCR using DNA purified from urine pellets collected by low-speed centrifugation yielded positive signals for patients in late acute as well as early phase who were positive by LAMP using plasma DNA or urine pellets. Among antibody-negative patients, 9.5% were positive by DNA detection. These findings indicate that the leptospirosis detection rate is increased by combining antibody and DNA detection, providing a new tool for timely diagnosis of infection.
KW - Antibody detection
KW - DNA detection
KW - Disaster
KW - Leptospirosis
KW - Urine
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U2 - 10.1016/j.diagmicrobio.2016.01.001
DO - 10.1016/j.diagmicrobio.2016.01.001
M3 - Article
C2 - 26860351
AN - SCOPUS:84960300894
VL - 84
SP - 287
EP - 291
JO - Diagnostic Microbiology and Infectious Disease
JF - Diagnostic Microbiology and Infectious Disease
SN - 0732-8893
IS - 4
ER -
