Combined antibody and DNA detection for early diagnosis of leptospirosis after a disaster

Hiroko Iwasaki, Haorile Chagan-Yasutan, Prisca Susan A. Leano, Nobuo Koizumi, Chie Nakajima, Delsi Taurustiati, Firmanto Hanan, Talitha Lea Lacuesta, Yugo Ashino, Yasuhiko Suzuki, Nina G. Gloriani, Elizabeth Freda O. Telan, Toshio Hattori

研究成果: Article査読

14 被引用数 (Scopus)

抄録

Early diagnosis based on laboratory confirmation is essential for managing leptospirosis. This study investigated the effectiveness of a novel method of detecting leptospirosis that combines measurement of anti-Leptospira antibodies by the microscopic agglutination test (MAT), enzyme-linked immunosorbent assay (ELISA), and immunochromatographic test (ICT) and leptospiral DNA by loop-mediated isothermal amplification (LAMP) and real-time PCR in plasma and 2 types of urine pellets. Of 113 suspected cases, 68.1%, 76.1%, and 60.2% were positive by MAT, ELISA, and ICT, respectively. Real-time PCR using DNA purified from urine pellets collected by low-speed centrifugation yielded positive signals for patients in late acute as well as early phase who were positive by LAMP using plasma DNA or urine pellets. Among antibody-negative patients, 9.5% were positive by DNA detection. These findings indicate that the leptospirosis detection rate is increased by combining antibody and DNA detection, providing a new tool for timely diagnosis of infection.

本文言語English
ページ(範囲)287-291
ページ数5
ジャーナルDiagnostic Microbiology and Infectious Disease
84
4
DOI
出版ステータスPublished - 2016 4 1
外部発表はい

ASJC Scopus subject areas

  • 微生物学(医療)
  • 感染症

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