Coexpression of mineralocorticoid receptors and 11 β-hydroxysteroid dehydrogenase 2 in human gastric mucosa

Katsuaki Kato, Hironobu Sasano, Shuichi Ohara, Hitoshi Sekine, Shizue Mochizuki, Tomoatsu Mune, Keigo Yasuda, Hiroshi Nagura, Tooru Shimosegawa, Takayoshi Toyota, Zygmunt Krozowski

研究成果: Article査読

13 被引用数 (Scopus)

抄録

The role of mineralocorticoids in human gastrointestinal tract is well established. In the stomach, aldosterone is thought to regulate electrolyte transport associated with gastric acid secretion. In mineralocorticoid target organs, the action of the glucocorticoid inactivating enzyme 11β-hydroxysteroid dehydrogenase type 2 (11β-HSD2) facilitates aldosterone binding to a nonselective mineralocorticoid receptor (MR) in the face of high levels of circulating glucocorticoids. In the present study, we examined 25 specimens of human stomach for the presence of MR and 11β-HSD2 using a [3H]aldosterone binding assay, Northern blot analysis, RT-PCR, and immunohistochemistry. Specific [3H]aldosterone binding sites were detected in gastric fundic mucosa, but not in the antrum. In fundic mucosa the K(d) was 0.72 ±0.05 nmol/L (mean ± SE), and B(max) was 6.0 ± 1.4 fmol per milligram of protein. Northern blot analysis demonstrated a faint band for MR mRNA at 6.0 kb, although message for 11β-HSD2 was undetectable. However, RT-PCR demonstrated specific PCR products for both MR and 11β-HSD2. Immunohistochemistry demonstrated the colocalization of MR and 11β-HSD2 only in parietal cells. MR-positive cells were further characterized by electron microscopy, confirming the identity of parietal cells. This study shows that parietal cells contain both MR and 11β-HSD2, suggesting that the human stomach is a novel target organ for mineralocorticoids. Aldosterone may, therefore, regulate biological functions of parietal cells including gastric acid secretion.

本文言語English
ページ(範囲)2568-2573
ページ数6
ジャーナルJournal of Clinical Endocrinology and Metabolism
84
7
DOI
出版ステータスPublished - 1999

ASJC Scopus subject areas

  • 内分泌学、糖尿病および代謝内科学
  • 生化学
  • 内分泌学
  • 臨床生化学
  • 生化学、医学

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