Coexistence of fibrotic and chondrogenic process in the capsule of idiopathic frozen shoulders

Y. Hagiwara, A. Ando, Y. Onoda, T. Takemura, T. Minowa, N. Hanagata, M. Tsuchiya, T. Watanabe, E. Chimoto, H. Suda, N. Takahashi, H. Sugaya, Y. Saijo, E. Itoi

研究成果: Article査読

38 被引用数 (Scopus)

抄録

Objective: To analyze changes in the capsule from idiopathic frozen shoulders and clarify their etiology. Materials and methods: Samples (the rotator interval capsule, middle glenohumeral ligament (MGHL), and inferior glenohumeral ligament (IGHL)) were collected from 12 idiopathic frozen shoulders with severe stiffness and 18 shoulders with rotator cuff tears as a control. The number of cells was counted and the tissue elasticity of the samples was calculated by scanning acoustic microscopy (SAM). The amount of glycosaminoglycan content was assessed by alcian blue staining. Gene and protein expressions related to fibrosis, inflammation, and chondrogenesis were analyzed by quantitative polymerase chain reaction (qPCR) and immunohistochemistry (IHC). Furthermore, the total genes of the two groups were compared by DNA microarray analysis. Results: The number of cells was significantly higher and the capsular tissue was significantly stiffer in idiopathic frozen shoulders compared with shoulders with rotator cuff tears. Staining intensity of alcian blue was significantly stronger in idiopathic frozen shoulders. Gene expressions related to fibrosis, inflammation, and chondrogenesis were significantly higher in idiopathic frozen shoulders compared with shoulders with rotator cuff tears assessed by both qPCR and DNA microarray analysis. Conclusion: In addition to fibrosis and inflammation, which used to be considered the main pathology of frozen shoulders, chondrogenesis is likely to have a critical role in pathogenesis of idiopathic frozen shoulders.

本文言語English
ページ(範囲)241-249
ページ数9
ジャーナルOsteoarthritis and Cartilage
20
3
DOI
出版ステータスPublished - 2012 3

ASJC Scopus subject areas

  • Rheumatology
  • Biomedical Engineering
  • Orthopedics and Sports Medicine

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