Cochlear expression of a dominant-negative GJB2R75W construct delivered through the round window membrane in mice

Yukihide Maeda, Kunihiro Fukushima, Akihiro Kawasaki, Kazunori Nishizaki, Richard J.H. Smith

研究成果: Article査読

34 被引用数 (Scopus)

抄録

Development of a gene-delivery method to the inner ear is an essential step for studies of hearing function and gene therapy. Application of liposomes or adenoviral vectors onto the intact round window membrane (RWM) offers the possibility of atraumatic exogenous gene transfer. GJB2 encodes the gap junction protein Connexin26, which plays a crucial role in potassium recycling in the inner ear. The R75W allele of GJB is a well-characterized mutation that causes deafness at the DFNA3 through a dominant-negative mechanism of action. In this study, a plasmid vector, pGJB2R75W-eGFP, was lipocomplexed with N-[1-(2,3-Dioleoloxy)propyl]N,N,N-trimethylammonium methylsulfate: cholesterol and applied onto mouse RWM. At 3 days (3 d) post-treatment, immunohistochemistry demonstrated GJB2R75W-eGFP transgene expression in the cochlea in: inner and outer pillar cells, outer hair cells, Claudius cells and, in the spiral limbus and ligament. Significant hearing loss was detected by auditory brainstem response testing after 1, 2 and 3 d post-treatment; hearing levels returned to control levels at 5 d post-treatment. These data confirm that GJB2R75W induces functional impairment in the mature cochlea through a dominant negative effect, and importantly, that RWM application of exogenous genes is a feasible method to test their impact on hearing.

本文言語English
ページ(範囲)250-254
ページ数5
ジャーナルNeuroscience Research
58
3
DOI
出版ステータスPublished - 2007 7
外部発表はい

ASJC Scopus subject areas

  • Neuroscience(all)

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