Co-repressor SMRT and class II histone deacetylases promote Bach2 nuclear retention and formation of nuclear foci that are responsible for local transcriptional repression

Hideto Hoshino, Tomonori George Nishino, Satoshi Tashiro, Masaya Miyazaki, Yoshihiro Ohmiya, Kazuhiko Igarashi, Sueharu Horinouchi, Minoru Yoshida

研究成果: Article

11 引用 (Scopus)

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Bach2 is a member of the BTB-basic region leucine zipper factor family and represses transcription activity directed by the TPA response element, the Maf recognition element (MARE) and the antioxidant-responsive element. Recently, it was reported that upon oxidative stress Bach2 forms nuclear foci surrounding the promyelocytic leukaemia (PML) bodies and specifically represses the transcription around the PML bodies. Here we report that expression of the silencing mediator of retinoid and thyroid receptor (SMRT) and histone deacetylase4 (HDAC4) enhances the formation of the Bach2 foci in the nuclear matrix. SMRT mediates the HDAC4 binding to Bach2, and HDAC4 facilitates the retention of Bach2 in the foci. Scratch transcription labelling and 3D-reconstruction from the confocal images demonstrated that transcription is suppressed in and around the Bach2 foci. Indeed, Bach2 bound MARE and repressed the expression from the chromosomally integrated MARE-driven reporter gene when co-expressed with SMRT and HDAC4. Our observations suggest that both SMRT and HDAC4 play an important role in nuclear retention and the Bach2 focus formation in the mammalian cell nucleus, which may contribute to the local transcription repression.

元の言語English
ページ(範囲)719-727
ページ数9
ジャーナルJournal of biochemistry
141
発行部数5
DOI
出版物ステータスPublished - 2007 5

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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