Cluster-forming property correlated with hemolytic activity by staphylococcal γ-hemolysin transmembrane pores

Noriko Tomita; Kazuyo Abe; Yoshiyuki Kamio; Makoto Ohta

doi:10.1016/j.febslet.2011.09.041

Cluster-forming property correlated with hemolytic activity by staphylococcal γ-hemolysin transmembrane pores

Noriko Tomita, Kazuyo Abe, Yoshiyuki Kamio, Makoto Ohta

流体科学研究所・流動創成研究部門

研究成果: Article › 査読

6 被引用数 (Scopus)

抄録

Staphylococcal γ-hemolysin (Hlg) is a pore-forming toxin consisting of two separate components, LukF (34 kDa) and Hlg2 (32 kDa). Here we show that Hlg pores aggregate and form clusters on human erythrocyte membranes in association with increasing hemolytic activity. Quantitative analysis using transmission electron microscopy and image processing revealed that the formation of single pores and clusters is related to the release of potassium ions and of hemoglobin from erythrocytes, respectively. This is the first study to suggest a novel and unique property which can facilitate hemolysis by the cluster formation of Hlg pores.

本文言語	English
ページ（範囲）	3452-3456
ページ数	5
ジャーナル	FEBS Letters
巻	585
号	21
DOI	https://doi.org/10.1016/j.febslet.2011.09.041
出版ステータス	Published - 2011 11 4

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

文献へのアクセス

10.1016/j.febslet.2011.09.041

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引用スタイル

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abstract = "Staphylococcal γ-hemolysin (Hlg) is a pore-forming toxin consisting of two separate components, LukF (34 kDa) and Hlg2 (32 kDa). Here we show that Hlg pores aggregate and form clusters on human erythrocyte membranes in association with increasing hemolytic activity. Quantitative analysis using transmission electron microscopy and image processing revealed that the formation of single pores and clusters is related to the release of potassium ions and of hemoglobin from erythrocytes, respectively. This is the first study to suggest a novel and unique property which can facilitate hemolysis by the cluster formation of Hlg pores.",

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author = "Noriko Tomita and Kazuyo Abe and Yoshiyuki Kamio and Makoto Ohta",

note = "Funding Information: This work was partially supported by a Grant-in-Aid for JSPS Fellows (RPD) and by a Global COE Program Grant of the World Center of Education and Research for Trans-disciplinary Flow Dynamics from Tohoku University. ",

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