Cloning, tissue expression, and mapping of a human photolyase homolog with similarity to plant blue-light receptors

P. J. Van Der Spek, K. Kobayashi, D. Bootsma, M. Takao, A. P.M. Eker, A. Yasui

研究成果: Article査読

66 被引用数 (Scopus)

抄録

Enzymatic photoreactivation is a DNA repair mechanism that removes UV- induced pyrimidine dimer lesions by action of a single enzyme, photolyase, and visible light. Its presence has been demonstrated in a wide variety of organisms, ranging from simple prokaryotes to higher eukaryotes. We have isolated a human gene encoding a 66-kDa protein that shows clear overall homology to known bacterial photolyase genes. The human gene product is more similar to plant blue-light receptors within class I photolyases than to higher eukaryote class II photolyases. Northern blot analysis showed two transcripts with constitutive expression in all tissues examined and an elevated expression in testis. In situ hybridization with a cDNA-derived probe localized this human gene to chromosome 12q23-q24.1. Southern analysis of the cloned human gene suggests a wide distribution of the gene family in various species.

本文言語English
ページ(範囲)177-182
ページ数6
ジャーナルGenomics
37
2
DOI
出版ステータスPublished - 1996 10 15

ASJC Scopus subject areas

  • 遺伝学

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