Cloning of cDNA encoding a new peptide C-terminal α-amidating enzyme having a putative membrane-spanning domain from Xenopus laevis skin

Kazuhiro Ohsuye, Katsuhiko Kitano, Yayoi Wada, Kayoko Fuchimura, Shoji Tanaka, Kensaku Mizuno, Hisayuki Matsuo

研究成果: Article査読

48 被引用数 (Scopus)

抄録

A cDNA clone encoding a precursor of a peptide C-terminal α-amidating enzyme (AE-I) from Xenopus laevis skin was recently isolated and sequenced in our laboratory. In this study, by using the restriction fragment of this clone as a hybridization probe, we have identified the cDNA encoding another new peptide C-terminal α-amidating enzyme (tentatively named AE-II) distinct from AE-I. The cDNA encodes a polypeptide of 875 amino acid residues, which contains a region extensively homologous to AE-I precursor at N-terminus. The encoded protein characteristically has a putative membrane-spanning domain near C-terminus. Our results indicate that C-terminal α-amide formation of peptides in Xenopus skin is regulated by at least two distinct α-amidating enzymes.

本文言語English
ページ(範囲)1275-1281
ページ数7
ジャーナルBiochemical and biophysical research communications
150
3
DOI
出版ステータスPublished - 1988 2月 15
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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