Cloning of γ-hexachlorocyclohexane dehydrochlorinase gene with its flanking regions from soil by activity-based screening techniques

Michihiro Ito, Akira Ono, Yoshiyuki Otsubo, Masataka Tsuda, Yuji Nagata

    研究成果: Article

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    Activity-based screening techniques were applied to clone a gene encoding γ-hexachlorocyclohexane (γ-HCH) dehydrochlorinase with its flanking regions from a cosmid-based library of DNA that was extracted from a γ-HCH-added suspension of HCH-contaminated soil. A total of 11 cosmid clones showing the γ-HCH dehydrochlorinase activity were obtained through the screenings, indicating the validity of this approach. All the clones had a linA gene identical to known one, but its flanking regions showed some structural variations with known ones, suggesting high likelihood of genetic divergence in the linA-flanking regions.

    元の言語English
    ページ(範囲)16-19
    ページ数4
    ジャーナルEuropean Journal of Soil Biology
    52
    DOI
    出版物ステータスPublished - 2012 9 1

    ASJC Scopus subject areas

    • Microbiology
    • Soil Science
    • Insect Science

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