Cloning and sequencing of a 2,5-dichlorohydroquinone reductive dehalogenase gene whose product is involved in degradation of γ- hexachlorocyclohexane by Sphingomonas paucimobilis

Keisuke Miyauchi, Seug Kyo Suh, Yuji Nagata, Masamichi Takagi

研究成果: Article査読

86 被引用数 (Scopus)

抄録

Sphingomonas (formerly Pseudomonas) paucimobilis UT26 utilizes Υ- hexachlorocyclohexane (γ-HCH), a halogenated organic insecticide, as a sole carbon and energy source. In a previous study, we showed that Υ-HCH is degraded to 2,5-dichlorohydroquinone (2,5-DCHQ) (Y. Nagata, R. Ohtomo, K. Miyauchi, M. Fukuda, K. Yano, and M. Takagi, J. Bacteriol. 176:3117-3125, 1994). In the present study, we cloned and characterized a gene, designated linD, directly involved in the degradation of 2,5-DCHQ. The linD gene encodes a peptide of 343 amino acids and has a low level of similarity to proteins which belong to the glutathione S-transferase family. When Lind was overproduced in Escherichia coli, a 40-kDa protein was found after sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Northern blot analysis revealed that expression of the linD gene was induced by 2,5-DCHQ in 8. paucimobilis UT26. Thin-layer chromatography and gas chromatography-mass spectrometry analyses with the LinD-overexpressing E. coli cells revealed that Lind converts 2,5-DCHQ rapidly to chlorohydroquinone (CHQ) and also converts CHQ slowly to hydroquinone. Lind activity in crude Cell extracts was increased 3.7-fold by the addition of glutathione. All three of the Tn5- induced mutants of UT26, which lack 2,5-DCHQ dehalogenase activity, had rearrangements or a deletion in the lind region. These results indicate that LinD is a glutathione-dependent reductive dehalogenase involved in the degradation of Υ-HCH by S. paucimobilis UT26.

本文言語English
ページ(範囲)1354-1359
ページ数6
ジャーナルJournal of bacteriology
180
6
DOI
出版ステータスPublished - 1998 3
外部発表はい

ASJC Scopus subject areas

  • 微生物学
  • 分子生物学

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