Cloning and nucleotide sequence of the genomic taka-amylase a gene of aspergillus oryzae

Setsuzo Tada, Yuzuru Iimura, Katsuya Gomi, Kojiro Takahashi, Shodo Hara, Kiyoshi Yoshizawa

研究成果: Article査読

104 被引用数 (Scopus)

抄録

The Taka-amylase A (TAA) gene was cloned from the genomic library of A. oryzae using synthetic DNA oligomers as probes. The gene was located in a 3.7 Kbp EcoRI fragment. A. oryzae transformants containing a EcoRl fragment showed increased TAA activity, the increase being 2 to 5fold. The complete nucleotide sequence of the gene was determined and it was found that the gene consisted of 2040 bp, with eight introns. The deduced amino acid sequence was compared with that reported by Toda et al., with the following findings. A presumed signal peptide consisting of twenty-one amino acids was found at the N-terminal. One insertion, one deletion and ten substitutions of amino acids were observed, which may be due to strain variation.

本文言語English
ページ(範囲)593-599
ページ数7
ジャーナルAgricultural and Biological Chemistry
53
3
DOI
出版ステータスPublished - 1989 1 1
外部発表はい

ASJC Scopus subject areas

  • 生化学、遺伝学、分子生物学(全般)
  • 農業および生物科学(全般)

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