cis-Regulatory inputs of the wnt8 gene in the sea urchin endomesoderm network

Takuya Minokawa, Athula H. Wikramanayake, Eric H. Davidson

研究成果: Article査読

80 被引用数 (Scopus)


Expression of the wnt8 gene is the key transcriptional motivator of an intercellular signaling loop which drives endomesoderm specification forward early in sea urchin embryogenesis. This gene was predicted by network perturbation analysis to be activated by inputs from the blimp1/krox gene, itself expressed zygotically in the endomesoderm during cleavage; and by a Tcf1/β-catenin input. The implication is that zygotic expression of wnt8 is stimulated in neighboring cells by its own gene product, since reception of the Wnt8 ligand causes β-catenin nuclearization. Here, the modular cis-regulatory system of the wnt8 gene of Strongylocentrotus purpuratus was characterized functionally, and shown to respond to blockade of both Blimp1/Krox and Tcf1/β-catenin inputs just as does the endogenous gene. The genomic target sites for these factors were demonstrated by mutation in one of the cis-regulatory modules. The Tcf1/β-catenin and Blimp1/Krox inputs are both necessary for normal endomesodermal expression mediated by this cis-regulatory module; thus, the genomic regulatory code underlying the predicted signaling loop thus resides in the wnt8 cis-regulatory sequence. In a second regulatory region, which initiates expression in micromere and macromere descendant cells early in cleavage, Tcf1 sites act to repress ectopic transcription in prospective ectoderm cells.

ジャーナルDevelopmental Biology
出版ステータスPublished - 2005 12 15

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

フィンガープリント 「cis-Regulatory inputs of the wnt8 gene in the sea urchin endomesoderm network」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。