ChlH, the H subunit of the Mg-chelatase, is an anti-sigma factor for SigE in Synechocystis sp. PCC 6803

Takashi Osanai, Masahiko Imashimizu, Asako Seki, Shusei Sato, Satoshi Tabata, Sousuke Imamura, Munehiko Asayama, Masahiko Ikeuchi, Kan Tanaka

研究成果: Article査読

51 被引用数 (Scopus)

抄録

Although regulation of sigma factors has been intensively investigated, anti-sigma factors have not been identified in oxygenic photosynthetic organisms. A previous study suggested that the sigma factor, SigE, of the cyanobacterium Synechocystis sp. PCC 6803, a positive regulator of sugar catabolism, is posttranslation-ally activated by light-to-dark transition. In the present study, we found that the Hsubunit of Mg-chelataseChlH interacts with sigma factor SigE by yeast two-hybrid screening, and immunoprecipita-tion analysis revealed that ChlH associates with SigE in a light-dependent manner in vivo. We also found that Mg2+ promotes the interaction of SigE and ChlH and determines their localization in vitro. In vitro transcription analysis demonstrated that ChlH inhibits the transcription activity of SigE. Based on these results, we propose a model in which ChlH functions as an anti-sigma factor, transducing light signals to SigE in a process mediated by Mg 2+.

本文言語English
ページ(範囲)6860-6865
ページ数6
ジャーナルProceedings of the National Academy of Sciences of the United States of America
106
16
DOI
出版ステータスPublished - 2009 4 21

ASJC Scopus subject areas

  • General

フィンガープリント 「ChlH, the H subunit of the Mg-chelatase, is an anti-sigma factor for SigE in Synechocystis sp. PCC 6803」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル