Chemical characterization of sialyl oligosaccharides isolated from tammar wallaby (Macropus eugenii) milk

Tadasu Urashima, Tadao Saito, Yuhnagi Tsuji, Yasuko Taneda, Toshihide Takasawa, Michael Messer

研究成果: Article査読

23 被引用数 (Scopus)

抄録

Two components of the sialyl oligosaccharides were separated from milk of the tammar wallaby (Macropus eugenii) by gel filtration and ion exchange chromatography. Their molecular weights, estimated by gel filtration on HPLC using 5 mM triethylamine-acetate buffer (pH 5.0), were approx. 3,000. Their monosaccharide compositions, determined by GC analysis after methanolysis and by colorimetric assay, were (Glc)1(Gal)9(GlcNAc)2(Neu5Ac)1, and (Glc)1(Gal)8(GlcNAc)2(Neu5Ac)2. Their chemical structures were further elucidated by 1H-NMR and methylation analysis. The results suggest that their approximate structures are: {A figure is presented}.

本文言語English
ページ(範囲)64-72
ページ数9
ジャーナルBBA - General Subjects
1200
1
DOI
出版ステータスPublished - 1994 5 25

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学

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