Characterization of two endoglucanases for the classification of the earthworm, Eisenia fetida Waki

Shin Ichi Akazawa, Yuki Ikarashi, Jun Yarimizu, Keisuke Yokoyama, Tomoya Kobayashi, Hikaru Nakazawa, Wataru Ogasawara, Yasushi Morikawa

研究成果: Article査読

3 被引用数 (Scopus)

抄録

Eisenia fetida and Eisenia andrei are vermicomposting species that are used as model animals for testing chemical material toxicology. Eisenia spp. are grown commercially in various fields in Japan. However, these two species have not been classified because it is difficult to distinguish them morphologically; thus, all bred earthworms are called E. fetida. However, it has been proposed that these two species have different expression regulation mechanisms. Here, we classified a sample of earthworms purchased from several farms, confirming that both E. fetida and E. andrei are present in Japanese earthworm breeding programs. We also characterized two highly active endoglucanases (EfEG1 and EfEG2) from the E. fetida Waki strain, which contained strong fibrinolytic enzymes for improving human health. We confirmed that EfEG1 is 1371 bp long and belongs to GHF9. Thus, E. fetida Waki may have commercial application for biomass utilization and as a dietary health supplement.

本文言語English
ページ(範囲)55-66
ページ数12
ジャーナルBioscience, Biotechnology and Biochemistry
80
1
DOI
出版ステータスPublished - 2016
外部発表はい

ASJC Scopus subject areas

  • バイオテクノロジー
  • 分析化学
  • 生化学
  • 応用微生物学とバイオテクノロジー
  • 分子生物学
  • 有機化学

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