Characterization of the Streptococcus mutans pyruvate formate-lyase (PFL)-activating enzyme gene by complementary reconstitution of the in vitro PFL-reactivating system

Yasuhito Yamamoto, Yutaka Sato, Shoko Takahashi-Abbe, Nobuhiro Takahashi, Harutoshi Kizaki

研究成果: Article査読

12 被引用数 (Scopus)

抄録

The act gene was identified and an act mutant as well as the pfl mutant was constructed in Streptococcus mutans. Pyruvate formate-lyase (PFL) activity was regenerated with the mixture of the respective cell extracts from these mutants by complementary reconstitution of the in vitro reactivating system. The S. mutans act gene encoded the sole enzyme able to activate the PFL protein in this organism.

本文言語English
ページ(範囲)4773-4777
ページ数5
ジャーナルInfection and immunity
68
8
DOI
出版ステータスPublished - 2000

ASJC Scopus subject areas

  • 寄生虫科
  • 微生物学
  • 免疫学
  • 感染症

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