抄録
Genomic DNA fragments containing the S3-, S4, and S6-RNase genes were isolated from the sweet cherry (Prunus avium L.) and sequenced. Comparison of the 5′-flanking sequences of these three S-RNases indicated that a highly conserved region (designated CR) existed just upstream from the putative TATA boxes. We postulate that CR contains cis-regulatory element(s) involved in pistil expression. To examine the activity of the isolated S-RNase promoters of sweet cherry in the pistil, we transiently introduced approximately 650-bp fragments of the S4- and S6-RNase promoters fused to β-glucuronidase (GUS) gene into the pistil of the petunia using a particle bombardment technique. Histochemical analysis showed that the 5′-flanking region of each S-RNase was active in the pistil. This suggests that cis-regulatory element(s) for pistil-specific expression may exist(s) within the 650-bp region upstream from the TATA box in the sweet cherry S-RNase promoter.
本文言語 | English |
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ページ(範囲) | 191-194 |
ページ数 | 4 |
ジャーナル | Genes and Genetic Systems |
巻 | 78 |
号 | 2 |
DOI | |
出版ステータス | Published - 2003 4月 |
外部発表 | はい |
ASJC Scopus subject areas
- 分子生物学
- 遺伝学