Characterization of the S-RNase promoters from sweet cherry (Prunus avium L.)

Takako Ishizaka, Hideaki Nakano, Takashi Suzuki, Hiroyasu Kitashiba

研究成果: Article査読

5 被引用数 (Scopus)

抄録

Genomic DNA fragments containing the S3-, S4, and S6-RNase genes were isolated from the sweet cherry (Prunus avium L.) and sequenced. Comparison of the 5′-flanking sequences of these three S-RNases indicated that a highly conserved region (designated CR) existed just upstream from the putative TATA boxes. We postulate that CR contains cis-regulatory element(s) involved in pistil expression. To examine the activity of the isolated S-RNase promoters of sweet cherry in the pistil, we transiently introduced approximately 650-bp fragments of the S4- and S6-RNase promoters fused to β-glucuronidase (GUS) gene into the pistil of the petunia using a particle bombardment technique. Histochemical analysis showed that the 5′-flanking region of each S-RNase was active in the pistil. This suggests that cis-regulatory element(s) for pistil-specific expression may exist(s) within the 650-bp region upstream from the TATA box in the sweet cherry S-RNase promoter.

本文言語English
ページ(範囲)191-194
ページ数4
ジャーナルGenes and Genetic Systems
78
2
DOI
出版ステータスPublished - 2003 4
外部発表はい

ASJC Scopus subject areas

  • 分子生物学
  • 遺伝学

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