Characterization of the promoter of the mouse preproorexin gene

Haruno Kato, Hiroshi Hosoda, Toru Fukuda, Shoichi Masushige, Satoshi Kida

研究成果: Article査読

3 被引用数 (Scopus)

抄録

The neuropeptide Orexin is involved in the regulation of the sleep-awake cycle and feeding behavior. We isolated a 22-kb genomic clone containing the 5′ flanking region of the mouse Orexin promoter. We determined that the transcription start site (+1) is located 96 nucleotides upstream of the initiation codon. The start site region contained consensus sequences corresponding to the transcription initiator and TATA box. Analysis of promoter activity using PC12 cells showed that regions between -13 and +112 and between -1,868 and -780 contained nerve growth factor (NGF)-responsive positive regulatory element and a negative regulatory element respectively.

本文言語English
ページ(範囲)840-843
ページ数4
ジャーナルBioscience, Biotechnology and Biochemistry
71
3
DOI
出版ステータスPublished - 2007
外部発表はい

ASJC Scopus subject areas

  • バイオテクノロジー
  • 分析化学
  • 生化学
  • 応用微生物学とバイオテクノロジー
  • 分子生物学
  • 有機化学

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