Characterization of the physicochemical properties of phospholipid vesicles prepared in CO2/water systems at high pressure

Hidemi Nakamura, Shogo Taguchi, Keishi Suga, Keita Hayashi, Ho Sup Jung, Hiroshi Umakoshi

研究成果: Article査読

4 被引用数 (Scopus)

抄録

Phospholipid vesicles were prepared by the nonsolvent method using high-pressure CO2/water systems. The membrane properties of vesicles prepared at different pressures and temperatures were mainly characterized based on analysis of the membrane fluidity and membrane polarity, using the fluorescent probes 1,6-diphenyl-1,3,5-hexatriene and 6-dodecanoyl-N,N-dimethyl-2-naphthylamine, respectively. The CO2(liquid)/water(liquid) and the CO2(supercritical)/water(liquid) two-phase (heterogeneous) systems resulted in the formation of vesicles with high yield (ca. 85%-88%). The membrane fluidity and polarity of the vesicles were similar to those of liposomes prepared by the conventional method. It is suggested that high-pressure CO2 can be used to form an appropriate hydrophobic-hydrophilic interface where phospholipid molecules as a self-assembled membrane.

本文言語English
論文番号031005
ページ(範囲)1-6
ページ数6
ジャーナルBiointerphases
10
3
DOI
出版ステータスPublished - 2015
外部発表はい

ASJC Scopus subject areas

  • 化学 (全般)
  • 生体材料
  • 材料科学(全般)
  • 生化学、遺伝学、分子生物学(全般)
  • 物理学および天文学(全般)

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