Characterization of the Gene Encoding the MPB51, One of the Major Secreted Protein Antigens of Mycobacterium bovis BCG, and Identification of the Secreted Protein Closely Related to the Fibronectin Binding 85 Complex

N. OHARA, H. KITAURA, H. HOTOKEZAKA, T. NISHIYAMA, N. WADA, S. MATSUMOTO, T. MATSUO, M. NAITO, T. YAMADA

研究成果: Article査読

50 被引用数 (Scopus)

抄録

The secreted protein MPB51 is one of the major proteins in the culture filtrate of Mycobacterium bovis BCG (BCG) and is a protein immunologically cross‐reacting with the fibronectin binding 85 complex secreted by this bacterium. The gene encoding MPB51 (mpb51) was cloned, sequenced, and expressed in Escherichia coll. The mpb51 gene was mapped downstream of the gene for 85A component with 179 bp spaces. The mpb51 gene encoded 299 amino acids, including 33 amino acids for the signal peptide, followed by 266 amino acids for the mature protein with a molecular mass of 27807.37 Da. This is the first complete sequence of MPB51. MPB51 showed 37–43% homology to the components of 85 complex. Two‐dimensional electrophoresis of culture fluids of BCG and Western blotting indicated the existence of the other novel protein(s) which strongly cross‐reacted with the α antigen (85B) and MPB51.

本文言語English
ページ(範囲)433-442
ページ数10
ジャーナルScandinavian Journal of Immunology
41
5
DOI
出版ステータスPublished - 1995 5
外部発表はい

ASJC Scopus subject areas

  • Immunology

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