Characterization of the amyR gene encoding a transcriptional activator for the amylase genes in Aspergillus nidulans

Shuji Tani, Yoko Katsuyama, Tomoko Hayashi, Hayato Suzuki, Masashi Kato, Katsuya Gomi, Tetsuo Kobayashi, Norihiro Tsukagoshi

研究成果: Article査読

68 被引用数 (Scopus)

抄録

The Aspergillus nidulans amyR gene and its cDNA were cloned and sequenced. The genomic gene comprised 2, 092 bp, interrupted by two short introns, and encoded a cys-6 zinc transcriptional activator (AMYR) of 662 amino acid residues with a calculated molecular mass of 72, 862 Da. Disruption of the amy R gene caused defects in the utilization of maltose and starch and abolished expression of the taaG2 gene encoding A. oryzae Taka-amylase A, which is inducibly and abundantly expressed in the wild-type A. nidulans. Expression of the amyR gene was under the control of the carbon catabolite repressor, CREA. The growth defect of the malA1 mutant on maltose was complemented by the amyR gene; and the amyR gene derived from the mutant possessed a single mutation, from A to T, at position 1, 483, resulting in a substitution of His478 to Leu. These results indicate that the amyR gene is identical to the genetically defined malA gene. AMYR possessed five domains (Zn and MH1-MH4) homologous to Ma163p, a transcriptional activator for the genes involved in maltose utilization in Saccharomyces cerevisiae. The His478 to Leu substitution lay within the MH3 domain, corresponding to the negative regulatory domain of Mal63p which relieves the inhibitory effect on the activation function in response to maltose.

本文言語English
ページ(範囲)10-15
ページ数6
ジャーナルCurrent Genetics
39
1
DOI
出版ステータスPublished - 2001

ASJC Scopus subject areas

  • 遺伝学

フィンガープリント

「Characterization of the amyR gene encoding a transcriptional activator for the amylase genes in Aspergillus nidulans」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル