Characterization of Monoclonal Antibodies against Human Parvovirus B19

Nobuo Yaegashi; Kohtaro Tada; Kinnya Nagata; Kazuo Sugamura

doi:10.1111/j.1348-0421.1989.tb02006.x

Characterization of Monoclonal Antibodies against Human Parvovirus B19

Nobuo Yaegashi, Kohtaro Tada, Kinnya Nagata, Kazuo Sugamura

医学系研究科・医科学専攻

研究成果: Article › 査読

26 被引用数 (Scopus)

抄録

Eleven hybridoma cell lines producing mouse monoclonal antibodies (mAbs) against human parvovirus B19 were established. Their specificity was as follows. Approximately 5% of fetal erythroid cells inoculated with B19 reacted with all the mAbs and with anti-B19 positive human serum, but not with negative serum by indirect double immunofluorescence staining. All the mAbs recognized both VP-1 (84 kDa) and VP-2 (58 kDa) capsid proteins of B19 virions propagated in vitro and in vivo by Western blotting, and immunoprecipitated B19 virions.

本文言語	English
ページ（範囲）	561-567
ページ数	7
ジャーナル	MICROBIOLOGY and IMMUNOLOGY
巻	33
号	7
DOI	https://doi.org/10.1111/j.1348-0421.1989.tb02006.x
出版ステータス	Published - 1989

ASJC Scopus subject areas

微生物学
免疫学
ウイルス学

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10.1111/j.1348-0421.1989.tb02006.x

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author = "Nobuo Yaegashi and Kohtaro Tada and Kinnya Nagata and Kazuo Sugamura",

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doi = "10.1111/j.1348-0421.1989.tb02006.x",

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AU - Yaegashi, Nobuo

AU - Tada, Kohtaro

AU - Nagata, Kinnya

AU - Sugamura, Kazuo

PY - 1989

Y1 - 1989

N2 - Eleven hybridoma cell lines producing mouse monoclonal antibodies (mAbs) against human parvovirus B19 were established. Their specificity was as follows. Approximately 5% of fetal erythroid cells inoculated with B19 reacted with all the mAbs and with anti-B19 positive human serum, but not with negative serum by indirect double immunofluorescence staining. All the mAbs recognized both VP-1 (84 kDa) and VP-2 (58 kDa) capsid proteins of B19 virions propagated in vitro and in vivo by Western blotting, and immunoprecipitated B19 virions.

AB - Eleven hybridoma cell lines producing mouse monoclonal antibodies (mAbs) against human parvovirus B19 were established. Their specificity was as follows. Approximately 5% of fetal erythroid cells inoculated with B19 reacted with all the mAbs and with anti-B19 positive human serum, but not with negative serum by indirect double immunofluorescence staining. All the mAbs recognized both VP-1 (84 kDa) and VP-2 (58 kDa) capsid proteins of B19 virions propagated in vitro and in vivo by Western blotting, and immunoprecipitated B19 virions.

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EP - 567

JO - Microbiology and Immunology

JF - Microbiology and Immunology

SN - 0385-5600

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Characterization of Monoclonal Antibodies against Human Parvovirus B19

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