Characterization of Lamprey Rhodopsin by Isolation from Lamprey Retina and Expression in Mammalian Cells

Osamu Hisatomi, Makoto Ishikawa, Akira Tonosaki, Fumio Tokunaga

研究成果: Article査読

12 被引用数 (Scopus)

抄録

A visual pigment was extracted from lamprey retina and was expressed in cultured mammalian cells (293S) using a cDNA fragment isolated from lamprey retina. The extracted pigment, a putative lamprey rhodopsin, had an absorption maximum at 503 nm. The recombinant lamprey rhodopsin, reconstituted with 11-cis-retinal, showed an absorption maximum at about 500 nm. Both pigments reacted with an anti-bovine rhodopsin antibody (Rh29), which recognizes the short photoreceptor cells in lamprey retina. Unlike rhodopsins of higher vertebrates, the lamprey rhodopsin bleached gradually in the presence of 100 mM hydroxylamine even in the dark. Our results suggest that, despite its high similarities with other vertebrate rhodopsins, lamprey rhodopsin has a character different from those of higher vertebrates.

本文言語English
ページ(範囲)792-795
ページ数4
ジャーナルPhotochemistry and Photobiology
66
6
DOI
出版ステータスPublished - 1997 12
外部発表はい

ASJC Scopus subject areas

  • 生化学
  • 物理化学および理論化学

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