TY - JOUR
T1 - Characterization of a precursor T-cell line (THP-6) with rearranged T-cell receptor β chain gene
AU - Minegishi, Masayoshi
AU - Tsuchiya, Shigeru
AU - Minegishi, Naoko
AU - Yoshie, Osamu
AU - Nakamura, Masataka
AU - Konno, Tasuke
N1 - Funding Information:
THE MAJORITY of human lymphoid leukemias and lymphomas are now known to originate in cells at various stages of maturation in T or B lineage and have contributed to our understanding of the development of T and B cells [l-3]. Through studies of T-cell leukemias and lymphomas, early thymocytes were thought to express CD7, CD5, T!9 (transferrin receptor) and often CD2 antigens [3]. Previously, we established a cell line (THP-6) from a 6-yr-old girl with acute lymphoblastic leukemia. THP-6 was originally thought to be a non-T/non-B cell line [4]. In the present study we further characterized THP-6 with respect to cell surface antigen expression as well as immunoglobulin (Ig) gene and T-cell receptor * This work was supported in part by a grant from Research Grant from Ministry of Education, Science and Culture, Japan.
Copyright:
Copyright 2015 Elsevier B.V., All rights reserved.
PY - 1988
Y1 - 1988
N2 - A previously established human leukemia cell line, designated THP-6, was further characterized with respect to cell surface antigen expression and immunoglobulin(Ig) and T-cell receptor(TCR) gene status. THP-6 cells were positive for CD7 and CD5 antigens and terminal deoxynucleotidyl transferase, but negative for CD2, CD1, CD4, CD8, CD10, cytoplasmic and surface CD3 and HLA-DR antigens, suggesting a precursor T-cell line. Analysis of Ig and TCR β chain genes revealed that THP-6 had a rearranged TCR β chain gene and a germline Ig gene. These results, in agreement with its phenotype, confirmed that THP-6 was of the T-cell lineage.
AB - A previously established human leukemia cell line, designated THP-6, was further characterized with respect to cell surface antigen expression and immunoglobulin(Ig) and T-cell receptor(TCR) gene status. THP-6 cells were positive for CD7 and CD5 antigens and terminal deoxynucleotidyl transferase, but negative for CD2, CD1, CD4, CD8, CD10, cytoplasmic and surface CD3 and HLA-DR antigens, suggesting a precursor T-cell line. Analysis of Ig and TCR β chain genes revealed that THP-6 had a rearranged TCR β chain gene and a germline Ig gene. These results, in agreement with its phenotype, confirmed that THP-6 was of the T-cell lineage.
KW - Precursor T cells
KW - TCR β chain
KW - gene rearrangement
KW - leukemia cell line
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U2 - 10.1016/0145-2126(88)90140-3
DO - 10.1016/0145-2126(88)90140-3
M3 - Article
C2 - 3130528
AN - SCOPUS:0023900517
VL - 12
SP - 227
EP - 232
JO - Leukemia Research
JF - Leukemia Research
SN - 0145-2126
IS - 3
ER -
