Characterization of a lysK gene as an argE homolog in Thermus thermophilus HB27

Junichi Miyazaki, Nobuyuki Kobashi, Tomoyuki Fujii, Makoto Nishiyama, Hisakazu Yamane

研究成果: Article査読

27 被引用数 (Scopus)

抄録

We conducted a chromosome walk to obtain a DNA fragment downstream of lysJ and found an argE homolog in a putative operon composed of lysJ-orfC-orfD-argE homologs. A knockout mutant of the argE homolog showed significantly slow growth on a minimal medium, and the growth was markedly improved by addition of lysine. We therefore termed this gene lysK. Purified LysK protein has deacetylating activities for both N2-acetyllysine and N2-acetylornithine at almost equal efficiency. These results suggest that lysK which may share an ancestor with argE functions not only for the lysine biosynthesis, but also for arginine biosynthesis in Thermus thermophilus.

本文言語English
ページ(範囲)269-274
ページ数6
ジャーナルFEBS Letters
512
1-3
DOI
出版ステータスPublished - 2002 2月 13
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 構造生物学
  • 生化学
  • 分子生物学
  • 遺伝学
  • 細胞生物学

フィンガープリント

「Characterization of a lysK gene as an argE homolog in Thermus thermophilus HB27」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル