Characterization and in vitro cytotoxic effect of adriamycin conjugated monoclonal antibody prepared against breast cancer cell line

Hisanori Ikegaki, Noriaki Ohuchi, Takashi Masuko, Shinya Suzuki, Yuko Harada, Susumu Satomi, Yoshiyuki Hashimoto

研究成果: Article査読

抄録

We have produced an adriamycin-conjugated monoclonal antibody, AM1, covalently linked by acid-labile cis-aconitic spacer. The immunoconjugate, cisaconitic adriamycin (cAA)-AM1, was confirmed to retain the binding activity against human breast cancer cell lines by flow cytometry. The immunoconjugate was shown to be internalized into antigen-positive cancer cells by flow cytometry analysis and high performance liquid chromatography. Antitumor effects of cAA-AM1 were assessed on human breast cancer and colon cancer cell lines, with inhibition of 3H-leucine uptakes to the cells, c A A-AM1 demonstrated a selective cytotoxicity to ZR-75-1 which was reactive with AM1, whereas it showed no antitumor effect on SW1116 cells which did not react with AM1. Free adriamycin demonstrated a nonselective cytotoxicity against both cell lines. AM1 alone and cAA-control IgM did not show any antitumor effect on ZR-75-1. These results suggest that cAA-AM1 retains binding activity and specificity against breast cancer cells in vitro.

本文言語English
ページ(範囲)85-92
ページ数8
ジャーナルBreast Cancer
4
2
DOI
出版ステータスPublished - 1997 7月
外部発表はい

ASJC Scopus subject areas

  • 腫瘍学
  • 放射線学、核医学およびイメージング
  • 薬理学(医学)

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