抄録
We reported that hydrogel nanoparticles (nanogels) were formed by the self-assembly of cholesterol group bearing pullulans (CHP). We synthesized amino group bearing CHP (CHP-NH2). CHP-NH2 formed monodisperse cationic nanogels (∼20 nm) in water. CHP-NH2 nanogels formed complexes with DNAs. In this study, we investigated the interaction between CHP-NH2 nanogels-plasmid DNA complexes and cells. We selected the plasmid DNA to express the green fluorescent protein (GFP) in mammalian cell, for use as a transfection marker. The cells that were incubated with CHP-NH2 nanogels-plasmid DNA complexes expressed GFP. This result indicates that CHP-NH2 nanogel could be used as efficient DNA carrier.
| 本文言語 | English |
|---|---|
| ページ数 | 1 |
| 出版ステータス | Published - 2005 12 1 |
| 外部発表 | はい |
| イベント | 54th SPSJ Annual Meeting 2005 - Yokohama, Japan 継続期間: 2005 5 25 → 2005 5 27 |
Other
| Other | 54th SPSJ Annual Meeting 2005 |
|---|---|
| Country | Japan |
| City | Yokohama |
| Period | 05/5/25 → 05/5/27 |
ASJC Scopus subject areas
- Engineering(all)