抄録
We reported that hydrogel nanoparticles (nanogels) were formed by the self-assembly of cholesterol group bearing pullulans (CHP). We synthesized amino group bearing CHP (CHP-NH2). CHP-NH2 formed monodisperse cationic nanogels (∼20 nm) in water. CHP-NH2 nanogels formed complexes with DNAs. In this study, we investigated the interaction between CHP-NH2 nanogels-plasmid DNA complexes and cells. We selected the plasmid DNA to express the green fluorescent protein (GFP) in mammalian cell, for use as a transfection marker. The cells that were incubated with CHP-NH2 nanogels-plasmid DNA complexes expressed GFP. This result indicates that CHP-NH2 nanogel could be used as efficient DNA carrier.
本文言語 | English |
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ページ数 | 1 |
出版ステータス | Published - 2005 12月 1 |
外部発表 | はい |
イベント | 54th SPSJ Annual Meeting 2005 - Yokohama, Japan 継続期間: 2005 5月 25 → 2005 5月 27 |
Other
Other | 54th SPSJ Annual Meeting 2005 |
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国/地域 | Japan |
City | Yokohama |
Period | 05/5/25 → 05/5/27 |
ASJC Scopus subject areas
- 工学(全般)