Characteristics of nanogel-DNA complexes

Shin Ichi Sawada, Naomi Miyazawa, Shin Ichiro M. Nomura, Kazunari Akiyoshi

研究成果: Paper査読

1 被引用数 (Scopus)

抄録

We reported that hydrogel nanoparticles (nanogels) were formed by the self-assembly of cholesterol group bearing pullulans (CHP). We synthesized amino group bearing CHP (CHP-NH2). CHP-NH2 formed monodisperse cationic nanogels (∼20 nm) in water. CHP-NH2 nanogels formed complexes with DNAs. In this study, we investigated the interaction between CHP-NH2 nanogels-plasmid DNA complexes and cells. We selected the plasmid DNA to express the green fluorescent protein (GFP) in mammalian cell, for use as a transfection marker. The cells that were incubated with CHP-NH2 nanogels-plasmid DNA complexes expressed GFP. This result indicates that CHP-NH2 nanogel could be used as efficient DNA carrier.

本文言語English
ページ数1
出版ステータスPublished - 2005 12 1
外部発表はい
イベント54th SPSJ Annual Meeting 2005 - Yokohama, Japan
継続期間: 2005 5 252005 5 27

Other

Other54th SPSJ Annual Meeting 2005
CountryJapan
CityYokohama
Period05/5/2505/5/27

ASJC Scopus subject areas

  • Engineering(all)

フィンガープリント 「Characteristics of nanogel-DNA complexes」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル