We reported that hydrogel nanoparticles (nanogels) were formed by the self-assembly of cholesterol group bearing pullulans (CHP). We synthesized amino group bearing CHP (CHP-NH2). CHP-NH2 formed monodisperse cationic nanogels (∼20 nm) in water. CHP-NH2 nanogels formed complexes with DNAs. In this study, we investigated the interaction between CHP-NH2 nanogels-plasmid DNA complexes and cells. We selected the plasmid DNA to express the green fluorescent protein (GFP) in mammalian cell, for use as a transfection marker. The cells that were incubated with CHP-NH2 nanogels-plasmid DNA complexes expressed GFP. This result indicates that CHP-NH2 nanogel could be used as efficient DNA carrier.
|出版ステータス||Published - 2005 12月 1|
|イベント||54th SPSJ Annual Meeting 2005 - Yokohama, Japan|
継続期間: 2005 5月 25 → 2005 5月 27
|Other||54th SPSJ Annual Meeting 2005|
|Period||05/5/25 → 05/5/27|
ASJC Scopus subject areas