Changes of DNA methylation in protooncogenes in the process of radiation-induced transformation of mouse m5S 1M cells in vitro

K. Yasuzawa, S. Kodama, M. Kato, Y. Uehara, R. Tawa, S. Hirose, M. S. Sasaki, S. Okada, T. Ono

研究成果: Article査読

6 被引用数 (Scopus)

抄録

To assess the importance of changes in DNA methylation in an X-ray-induced cellular transformation process, methylation patterns of five nuclear protooncogenes in fifteen transformant clones were studied and compared to that of the parental non-transformed cell line m5S 1M. All transformants examined revealed an alteration in DNA methylation in some of the genes, although these changes were variable among them. A comparison of cellular characteristics with corresponding DNA methylation changes in different clones suggested that the loss of contact inhibition and the gain of anchorage independency were associated with increases of methylation in many genes, whereas the acquisition of tumorigenicity was often accompanied by a decrease of methylation in the N-myc and c-myc genes. Resultant data indicate that the alteration of DNA methylation is closely related to transformation process, yet how this involvement occurs is complex and remains unclear.

本文言語English
ページ(範囲)157-166
ページ数10
ジャーナルCancer Letters
67
2-3
DOI
出版ステータスPublished - 1992 12 24

ASJC Scopus subject areas

  • 腫瘍学
  • 癌研究

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