Change in hepatic handling of hepatocyte growth factor during liver regeneration in rats

K. X. Liu, Y. Kato, T. Terasaki, T. Nakamura, Y. Sugiyama

研究成果: Article査読

12 被引用数 (Scopus)

抄録

The change in the hepatic uptake clearance of hepatocyte growth factor (HGF) during liver regeneration in rats was examined. The hepatic uptake clearance per gram liver of 125I-labeled HGF in vivo decreased to a minimum level only 15 min after partial hepatectomy (PH). In the perfused liver, in which the effect of endogenous substances can be ignored, the hepatic uptake clearance of 125I-HGF did not change until at least 3 h after PH, suggesting that the decrease in the clearance in vivo is caused by some endogenous inhibitors. Such a decrease in the clearance cannot be explained only by the elevated endogenous HGF concentration in plasma. In addition, at 48 h after PH the decrease in hepatic uptake clearance was comparable for in vivo and perfused liver (~50 and 60% reduction, respectively), suggesting that the decreased hepatic clearance in viva at that time was caused mainly by reduction in the binding and/or uptake by the liver itself. In addition, the hepatic uptake clearance of 125I-HGF at that time in the presence of an excess (135 pM) of unlabeled HGF was comparable with that of the control, indicating that only the saturable portion of hepatic uptake clearance is reduced. The internalization rate constant representing the probability of cell surface-bound 125I-HGF being internalized per unit time increased to three times that of the control at 15 min after PH, demonstrating that the internalization of HGF is enhanced immediately after the start of liver regeneration.

本文言語English
ページ(範囲)G745-G753
ジャーナルAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
269
5 32-5
DOI
出版ステータスPublished - 1995

ASJC Scopus subject areas

  • 生理学
  • 肝臓学
  • 消化器病学
  • 生理学(医学)

フィンガープリント

「Change in hepatic handling of hepatocyte growth factor during liver regeneration in rats」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル