Cerebral alterations in a MPTP-mouse model of Parkinson's disease - An immunocytochemical study

Y. Muramatsu, R. Kurosaki, H. Watanabe, M. Michimata, M. Matsubara, Y. Imai, T. Araki

研究成果: Article査読

51 被引用数 (Scopus)

抄録

We investigated the immunohistochemical alterations of neuronal nitric oxide synthase (nNOS), endothelial NOS (eNOS), tyrosine hydroxylase (TH), microtuble-associated protein 2a,b (MAP 2), glial fibrillary acidic protein (GFAP), parvalbumin (PV), and dopamine transporter (DAT) in the striatum and substantia nigra following the application of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) in mice. TH-, MAP 2- and DAT-immunoreactive cells were decreased gradually in the striatum and substantia nigra from 1 day up to 7 days after MPTP treatment, as well as the reduction of the striatal dopamine, DOPAC and HVA content. The number of GFAP-immunoreactive astrocytes increased gradually in the striatum and substantia nigra from 1 day up to 7 days after MPTP treatment. Striatal nNOS-immunoreactive cells were unchanged in MPTP-treated mice. In the substantia nigra, intense immunoreactivity of nNOS-positive cells increased 5 hr after MPTP treatment. Thereafter, the immunoreactivity of nNOS-positive cells decreased gradually from 1 day up to 7 days after MPTP treatment. eNOS-immunopositive cells were unchanged in the striatum and substantia nigra. These results demonstrate that nNOS may play a key role in the development of MPTP neurotoxicity. Our findings also indicate that MPTP can cause the functional damage of interneurons in the substantia nigra, but not in the striatum.

本文言語English
ページ(範囲)1129-1144
ページ数16
ジャーナルJournal of Neural Transmission
110
10
DOI
出版ステータスPublished - 2003 10

ASJC Scopus subject areas

  • 神経学
  • 臨床神経学
  • 精神医学および精神衛生
  • 生物学的精神医学

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