Centromere-targeted de novo integrations of an LTR retrotransposon of Arabidopsis lyrata

Sayuri Tsukahara, Akira Kawabe, Akie Kobayashi, Tasuku Ito, Tomoyuki Aizu, Tadasu Shin-i, Atsushi Toyoda, Asao Fujiyama, Yoshiaki Tarutani, Tetsuji Kakutani

    研究成果: Article査読

    57 被引用数 (Scopus)

    抄録

    The plant genome evolves with rapid proliferation of LTR-type retrotransposons, which is associated with their clustered accumulation in gene-poor regions, such as centromeres. Despite their major role for plant genome evolution, no mobile LTR element with targeted integration into gene-poor regions has been identified in plants. Here, we report such targeted integrations de novo. We and others have previously shown that an ATCOPIA93 family retrotransposon in Arabidopsis thaliana is mobilized when the DNA methylation machinery is compromised. Although ATCOPIA93 family elements are low copy number in the wild-type A. thaliana genome, high-copynumber related elements are found in the wild-type Arabidopsis lyrata genome, and they show centromere-specific localization. To understand the mechanisms for the clustered accumulation of the A. lyrata elements directly, we introduced one of them, named Tal1 (Transposon of Arabidopsis lyrata 1), into A. thaliana by transformation. The introduced Tal1 was retrotransposed in A. thaliana, and most of the retrotransposed copies were found in centromeric repeats of A. thaliana, suggesting targeted integration. The targeted integration is especially surprising because the centromeric repeat sequences differ considerably between A. lyrata and A. thaliana. Our results revealed unexpectedly dynamic controls for evolution of the transposon-rich heterochromatic regions.

    本文言語English
    ページ(範囲)705-713
    ページ数9
    ジャーナルGenes and Development
    26
    7
    DOI
    出版ステータスPublished - 2012 4月 1

    ASJC Scopus subject areas

    • 遺伝学
    • 発生生物学

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