Centrifuge-based microfluidic device for trapping single cells in a drop

Hiromasa Hasegawa, Shotaro Yoshida, Yuya Morimoto, Shoji Takeuchi

研究成果: Conference contribution

抄録

We describe a centrifuge-based dynamic microarray system for trapping an array of single cells from small amount of suspension. We optimized microchannel designs and centrifugal force for minimizing sample volumes and maximizing trapping efficiencies, and achieved single-cell trapping by introducing only 5 μl of cell suspension with the centrifugal force of 70 G. Trapping of cells stained with DAPI shows that single cells were trapped at desired trapping spots. This system will be applicable for single cell assays using rare or expensive cells.

本文言語English
ホスト出版物のタイトル18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
出版社Chemical and Biological Microsystems Society
ページ1410-1412
ページ数3
ISBN(電子版)9780979806476
出版ステータスPublished - 2014 1 1
外部発表はい
イベント18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States
継続期間: 2014 10 262014 10 30

Other

Other18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
国/地域United States
CitySan Antonio
Period14/10/2614/10/30

ASJC Scopus subject areas

  • 制御およびシステム工学

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