Cellulase-catalysed glycosylation reactions: Simple route towards a highly selective synthesis of oligosaccharides

Olaf Karthaus, Shin Ichiro Shoda, Hiroshi Takano, Kei Obata, Shiro Kobayashi

研究成果: Article査読

41 被引用数 (Scopus)

抄録

Cellulase (EC 3.2.1.4) can be used for the enzyme-catalysed synthesis of oligosaccharides in a mixed buffer/organic solvent system. Besides glucopyranosides and cellooligosaccharides, non-glucosidic saccharides and modified glucoses as glycosyl acceptors also react with β-lactosyl fluoride as glycosyl donor. The reaction proceeds in a completely stereo- and regio-specific manner, giving rise to coupling products having β(1→4) glycosidic bonds exclusively. Based on these results, a model for the active site of cellulase (Trichoderma viride) and the orientation of the donor and acceptor therein is proposed.

本文言語English
ページ(範囲)1851-1857
ページ数7
ジャーナルJournal of the Chemical Society, Perkin Transactions 1
13
DOI
出版ステータスPublished - 1994

ASJC Scopus subject areas

  • 化学 (全般)

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