Expression, purification, and characterization of single-species of membrane protein are one of the most important technologies in post-genome era. Especially membrane proteins are difficult to isolate and purify with keeping their function. We engaged to study direct reconstitution of cell-free synthesized membrane proteins to giant liposomes. Recently we reported that the spontaneous formation of the three-dimensional lipid tubular network in the presence of various gangliosides or cholesterol. Here we report the experimental trial to obtain the fixed liposome-network with functional membrane proteins. As a model membrane protein, cytochrome-b5 was adopted. In the presence of liposomes, the expressed membrane proteins were directly and effectively displayed on the liposomal surface without aggregation. The proteo-liposomes were fixed to tubular-connected networks by adding gangliosides. The network formation process in the agarose gel was directly observed by fluorescent microscopy. Hemispheric liposomes changed their form to the tubular-networks in the gelation process. The stable lipid nanotube network can be used for constructing a biochemical micro-reactor to analyze membrane protein functions.
|出版ステータス||Published - 2006 12 1|
|イベント||55th Society of Polymer Science Japan Symposium on Macromolecules - Toyama, Japan|
継続期間: 2006 9 20 → 2006 9 22
|Other||55th Society of Polymer Science Japan Symposium on Macromolecules|
|Period||06/9/20 → 06/9/22|
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