Cellularmembrane-like structure of tubular-linked liposome network

Shin Ichiro M. Nomura, Kazunari Akiyoshi

研究成果: Paper査読


Expression, purification, and characterization of single-species of membrane protein are one of the most important technologies in post-genome era. Especially membrane proteins are difficult to isolate and purify with keeping their function. We engaged to study direct reconstitution of cell-free synthesized membrane proteins to giant liposomes. Recently we reported that the spontaneous formation of the three-dimensional lipid tubular network in the presence of various gangliosides or cholesterol. Here we report the experimental trial to obtain the fixed liposome-network with functional membrane proteins. As a model membrane protein, cytochrome-b5 was adopted. In the presence of liposomes, the expressed membrane proteins were directly and effectively displayed on the liposomal surface without aggregation. The proteo-liposomes were fixed to tubular-connected networks by adding gangliosides. The network formation process in the agarose gel was directly observed by fluorescent microscopy. Hemispheric liposomes changed their form to the tubular-networks in the gelation process. The stable lipid nanotube network can be used for constructing a biochemical micro-reactor to analyze membrane protein functions.

出版ステータスPublished - 2006 12 1
イベント55th Society of Polymer Science Japan Symposium on Macromolecules - Toyama, Japan
継続期間: 2006 9 202006 9 22


Other55th Society of Polymer Science Japan Symposium on Macromolecules

ASJC Scopus subject areas

  • 工学(全般)


「Cellularmembrane-like structure of tubular-linked liposome network」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。