Cellular conservation of endangered midget buffalo (Lowland Anoa, Bubalus quarlesi) by establishment of primary cultured cell, and its immortalization with expression of cell cycle regulators

Tomokazu Fukuda, Yuuka Iino, Takahiro Eitsuka, Manabu Onuma, Masafumi Katayama, Koichi Murata, Miho Inoue-Murayama, Kumiko Hara, Emiko Isogai, Tohru Kiyono

    研究成果: Article査読

    17 被引用数 (Scopus)

    抄録

    Lowland Anoa has become endangered due to hunting and human activity. Protection and breeding of endangered species in a controlled environment is the best way of conservation. However, it is not possible to adopt this approach for all endangered species because of the cost involved and the ever-increasing number of critically endangered species. In consideration of these limitations to the conventional conservation methods, we established a primary cell culture of endangered buffalo (Lowland Anoa, Bubalus quarlesi), for the preservation of this biological resource. In addition, we introduced human derived, mutant cyclin dependent kinase 4 (CDK4), Cyclin D, and telomerase reverse transcriptase (TERT) into the primary cells. The successful introduction of these three genes was confirmed by western blot with specific antibodies, and enzymatic activity. We also showed that the expression of mutant CDK4, Cyclin D, and TERT allows us to efficiently establish an immortalized cell line, with an intact chromosome pattern, from Lowland Anoa. To the best of our knowledge, this study is the first investigation that established an immortalized cell line of an endangered wild animal species.

    本文言語English
    ページ(範囲)1937-1947
    ページ数11
    ジャーナルCytotechnology
    68
    5
    DOI
    出版ステータスPublished - 2016 10月 1

    ASJC Scopus subject areas

    • バイオテクノロジー
    • バイオエンジニアリング
    • 生体医工学
    • 臨床生化学
    • 細胞生物学

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