A finely dispersed cell suspension was obtained from anther-derived rice callus cultured in AA medium, which contained an amino acid mixture as the sole nitrogen source. AA medium promoted the release of protoplasts effectively. These protoplasts, when cultured initially in B5 medium for 8-10 days followed by transfer to AA medium, showed sustained cell division and gave rise to callus with a high frequency. The critical factor in the protoplast culture seemed to be the nitrogen source in the culture medium. Green-spot and root formation could be obtained from the protoplast-derived callus.
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