Cell sheet technology for cardiac tissue engineering

Yuji Haraguchi, Tatsuya Shimizu, Katsuhisa Matsuura, Hidekazu Sekine, Nobuyuki Tanaka, Kenjiro Tadakuma, Masayuki Yamato, Makoto Kaneko, Teruo Okano

研究成果: Chapter

18 被引用数 (Scopus)

抄録

In this chapter, we describe the methods for the fabrication and transfer/transplantation of 3D tissues by using cell sheet technology for cardiac tissue regeneration. A temperature-responsive culture surface can be fabricated by grafting a temperature-responsive polymer, poly(N- isopropylacrylamide), onto a polystyrene cell culture surface. Cells cultured confluently on such a culture surface can be recovered as an intact cell sheet, and functional three-dimensional (3D) tissues can then be easily fabricated by layering the recovered cell sheets without any scaffolds or complicated manipulation. Cardiac cell sheets, myoblast sheets, mesenchymal stem cell sheets, cardiac progenitor cell sheets, etc., which are prepared from temperature-responsive culture surfaces, can be easily transplanted onto heart tissues of animal models, and those cell sheet constructs enhance the cell transplant efficiency, resulting in the induction of effective therapy.

本文言語English
ホスト出版物のタイトルCardiac Tissue Engineering
ホスト出版物のサブタイトルMethods and Protocols
出版社Humana Press Inc.
ページ139-155
ページ数17
ISBN(印刷版)9781493910465
DOI
出版ステータスPublished - 2014
外部発表はい

出版物シリーズ

名前Methods in Molecular Biology
1181
ISSN(印刷版)1064-3745

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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