Cell delivery using micropatterned ultra-thin polymeric films

We developed micropatterned polymeric ultra-thin films (nanosheets) consisting of biodegradable poly(lactic-co-glycolic acid) (PLGA) and magnetic nanoparticles (MNPs), on which monolayer of retinal pigment epithelial (RPE) cells were formed. The viability of RPE cells on the micropatterned nanosheets were evaluated along the syringe manipulation equipped with a clinical needle. Finally, subretinal injectability of the macropatterned nanosheets was demonstrated by using ex vivo swine eyes in terms of handleability and physical stability. The micropatterned nanosheets injectable by clinical syringe hold a great promise to transplant functional RPE cells minimum invasively.