Cell-based electrochemical assay for endotoxin using a secreted alkaline phosphatase reporter system

研究成果: Article査読

13 被引用数 (Scopus)

抄録

We developed a cell-based assay device for the detection of endotoxin, the potentially toxic compound that induces septic shock. Genetically-engineered cells that secrete alkaline phosphatase (SEAP) on exposure to endotoxin were cultured in an electrochemical cell device in medium containing p-aminophenyl phosphate and various concentrations of endotoxin. After 24 hr incubation, p-aminophenol (pAP), generated by SEAP-catalyzed hydrolysis, was detected by amperometry at + 0.35 V. The amperometric response increased with the concentration of endotoxin in the range of 0.01-1 ng/ml.

本文言語English
ページ(範囲)525-528
ページ数4
ジャーナルElectrochemistry
76
8
DOI
出版ステータスPublished - 2008 8

ASJC Scopus subject areas

  • Electrochemistry

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