Cell-associated collagenolytic activity by Candida albicans

Masahiro Nishimura, Hiroki Nikawa, Hirofumi Yamashiro, Haruki Nishimura, Taizo Hamada, Graham Embery

研究成果: Article査読

13 被引用数 (Scopus)

抄録

Cell associated collagenolytic activity of Candida albicans was quantified by measuring the degradation of synthetic peptide 2-furanacryloyl-Leu-Gly-Pro-Ala (FALGPA), which is a specific substrate for collagenase, by the freeze-thaw procedure method. This collagenolytic activity was enhanced by cells cultured in the presence of bovine serum albumin (BSA) in culture medium. However, this activity was inhibited in the presence of ethylenediaminetetraacetic acid disodium salt (EDTA-2Na), but not by the serine proteinase inhibitor p-amidinophenyl methanesulfonyl fluoride (APMSF), nor the aspartyl proteinase inhibitor pepstatin A. These results suggested the presence of a metalloenzyme on pericellular C. albicans.

本文言語English
ページ(範囲)125-128
ページ数4
ジャーナルMycopathologia
153
3
DOI
出版ステータスPublished - 2002
外部発表はい

ASJC Scopus subject areas

  • 微生物学
  • 応用微生物学とバイオテクノロジー
  • 農業および作物学
  • 獣医学(その他)

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