Cauliflower mosaic virus Tav protein induces leaf chlorosis in transgenic tobacco through a host response to virulence function of Tav

Sumyya Waliullah, Naomi Kosaka, Takashi Yaeno, Md Emran Ali, Ken Taro Sekine, Go Atsumi, Naoto Yamaoka, Masamichi Nishiguchi, Hideki Takahashi, Kappei Kobayashi

    研究成果: Article査読

    5 被引用数 (Scopus)

    抄録

    To study the precise mechanisms underlying the chlorosis caused by plant viruses, we previously established a synchronous experimental system using transgenic plants expressing Cauliflower mosaic virus multifunctional protein, Tav (transactivator/viroplasmin), under the control of an artificially inducible promoter. Shortly after the induction of Tav expression, pathogenesis-related protein (PR) 1a gene expression is upregulated in the transgenic tobacco lines, which show visible chlorosis within a week. The present study showed that the expression of Tav also induces some salicylic acid (SA)- and ethylene-responsive PR genes. In contrast to transiently expressed Tav, which suppressed Agrobacterium-induced and SA-induced PR1a expression, the artificial induction of Tav from the transgene did not affect SA-induced PR1a expression, rather it alone induced PR1a expression. In a deletion analysis, chlorosis and PR1a induction function in transgenic tobacco were mapped to a region in Tav that had been shown to have a role in pathogenesis in a susceptible host, elicitation of the hypersensitive response in a resistant host, suppression of RNA silencing, and the suppression of Tomato bushy stunt virus P19-mediated cell death in tobacco. The results suggest that Tav-induced chlorosis results from a host response, which accompanies PR1a induction, to pathogenic function of Tav.

    本文言語English
    ページ(範囲)261-270
    ページ数10
    ジャーナルJournal of General Plant Pathology
    81
    4
    DOI
    出版ステータスPublished - 2015 7月 23

    ASJC Scopus subject areas

    • 農業および作物学
    • 植物科学

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