Carbohydrate recognition mechanism of HA70 from Clostridium botulinum deduced from X-ray structures in complexes with sialylated oligosaccharides

Satoshi Yamashita, Hiromi Yoshida, Noboru Uchiyama, Yukari Nakakita, Shin Ichi Nakakita, Takashi Tonozuka, Keiji Oguma, Atsushi Nishikawa, Shigehiro Kamitori

研究成果: Article査読

26 被引用数 (Scopus)

抄録

Clostridium botulinum produces the botulinum neurotoxin, forming a large complex as progenitor toxins in association with non-toxic non-hemagglutinin and/or several different hemagglutinin (HA) subcomponents, HA33, HA17 and HA70, which bind to carbohydrate of glycoproteins from epithelial cells in the infection process. To elucidate the carbohydrate recognition mechanism of HA70, X-ray structures of HA70 from type C toxin (HA70/C) in complexes with sialylated oligosaccharides were determined, and a binding assay by the glycoconjugate microarray was performed. These results suggested that HA70/C can recognize both α2-3- and α2-6-sialylated oligosaccharides, and that it has a higher affinity for α2-3-sialylated oligosaccharides. Crown

本文言語English
ページ(範囲)2404-2410
ページ数7
ジャーナルFEBS Letters
586
16
DOI
出版ステータスPublished - 2012 7 30

ASJC Scopus subject areas

  • 生物理学
  • 構造生物学
  • 生化学
  • 分子生物学
  • 遺伝学
  • 細胞生物学

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