Capillary electrophoretic separation of humic substances using hydroxyethyl cellulose as a buffer additive and its application to characterization of humic substances in a river water sample

Toru Takahashi, Jun Kawana, Hitoshi Hoshino

研究成果: Article査読

3 被引用数 (Scopus)

抄録

We have developed a concise tool for the investigation of the transition of humic substances in environmental water. The separation of water-soluble humic substances was achieved rapidly and effectively by capillary electrophoresis using a polyacrylamide-coated capillary and a phosphate electrophoretic buffer solution (pH 7.0) containing hydroxyethyl cellulose. The separation mechanism was assessed using the ultrafiltration technique. The effect of the complexation of humic substances with metal ions was studied by using the proposed method. When Fe(III) ions or EDTA was added to the sample solution of fulvic acid, a distinct change in the electropherogram pattern based on the conformational change of fulvic acid was observed. The successful application of the proposed method to the characterization of humic substances in a river water sample was also demonstrated.

本文言語English
ページ(範囲)761-766
ページ数6
ジャーナルAnalytical and Bioanalytical Chemistry
393
2
DOI
出版ステータスPublished - 2009 1 1

ASJC Scopus subject areas

  • 分析化学
  • 生化学

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