Calmodulin-like activity associated with chromatin from pea buds

Hideaki Matsumoto, Motokazu Tanigawa, Yamaya Tomoyuki

研究成果: Article査読

20 被引用数 (Scopus)

抄録

NAD kinase and cyclic AMP phosphodiesterase were activated by a factor prepared from pea chromatin. About 62% of the original amount of the factor in the purified chromatin was recovered in the reassociated chromatin.The NAD kinase- and cyclic AMP phosphodiesterase-activating factor was released from the chromatin by heat treatment with ethylene glycol-bis(β-aminoethyl ether)- N,N,N',N'-tetraacetic acid (EGTA) then adsorbed on an affinity gel of phenothiazineagarose derivatives in the presence of excess Ca2+ over EGTA, after which it was eluted by a flush of EGTA. Activation of NAD kinase and cyclic AMP phosphodiesterase by this factor depended on the presence of Ca2+.The NAD kinase-activating factor and chromatin were coeluted when soluble chromatin was applied to a Bio-Gel A50 column. When chromatin was chromatographed on the same column after digestion by DNase I, the factor was eluted in association with the digested products of the chromatin. The activation properties of this factor indicate that a calmodulin-like activity exists in association with pea chromatin.The activation curves of cyclic AMP phosphodiesterase with the pea bud factor and with bovine brain calmodulin were compared. The amount of the factor in the chromatin fraction that corresponded to authentic calmodulin was calculated as 5.7 μg per mg DNA.

本文言語English
ページ(範囲)593-602
ページ数10
ジャーナルPlant and Cell Physiology
24
4
DOI
出版ステータスPublished - 1983 6
外部発表はい

ASJC Scopus subject areas

  • 生理学
  • 植物科学
  • 細胞生物学

フィンガープリント

「Calmodulin-like activity associated with chromatin from pea buds」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル