Caenorhabditis elegans RBX1 is essential for meiosis, mitotic chromosomal condensation and segregation, and cytokinesis

Yohei Sasagawa, Takeshi Urano, Yuji Kohara, Hideyuki Takahashi, Atsushi Higashitani

研究成果: Article査読

31 被引用数 (Scopus)

抄録

Background: The RING-H2 finger protein RBX1 (ROC1/HRT1) is a common subunit of SKP1-CDC53/CUL1-F-box (SCF), other cullins and von Hippel-Lindau (VHL) tumour suppressor E3 ubiquitin ligase complexes. RBX1 protein sequences are highly conserved in various species, including yeasts, Drosophila melanogaster, mice and humans. In Saccharomyces cerevisiae, RBX1 is essential for the G1/S transition. Results: Caenorhabditis elegans RBX1 is strongly expressed in early embryos and in the gonad, including meiotic cells. Depletion of RBX1 by RNA-mediated interference (RNAi) caused pronounced defects in the first meiotic division. Several irregular phenotypes were identified in embryos that escaped from meiotic arrest: defects in mitotic chromosomal condensation and segregation, abnormal chromosome bridges, giant nuclei, abnormal cortical protrusion, multinucleate cells and defects in germ cell proliferation. Moreover, histone H3 phosphorylation at Ser10 and Ser28 was significantly reduced in these embryos. The histone H3 phosphorylation defect of embryos was rescued by the additional depletion of protein phosphatase 1 (GLC7α/β) by RNAi. Conclusion: These results indicate that the RBX1 protein participates in diverse functions relevant to chromosome metabolism and cell cycle control.

本文言語English
ページ(範囲)857-872
ページ数16
ジャーナルGenes to Cells
8
11
DOI
出版ステータスPublished - 2003 10

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

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