Cadherin-based adhesions in the apical endfoot are required for active Notch signaling to control neurogenesis in vertebrates

Jun Hatakeyama, Yoshio Wakamatsu, Akira Nagafuchi, Ryoichiro Kageyama, Ryuichi Shigemoto, Kenji Shimamura

研究成果: Article査読

59 被引用数 (Scopus)

抄録

The development of the vertebrate brain requires an exquisite balance between proliferation and differentiation of neural progenitors. Notch signaling plays a pivotal role in regulating this balance, yet the interaction between signaling and receiving cells remains poorly understood. We have found that numerous nascent neurons and/or intermediate neurogenic progenitors expressing the ligand of Notch retain apical endfeet transiently at the ventricular lumen that form adherens junctions (AJs) with the endfeet of progenitors. Forced detachment of the apical endfeet of those differentiating cells by disrupting AJs resulted in precocious neurogenesis that was preceded by the downregulation of Notch signaling. Both Notch1 and its ligand Dll1 are distributed around AJs in the apical endfeet, and these proteins physically interact with ZO-1, a constituent of the AJ. Furthermore, live imaging of a fluorescently tagged Notch1 demonstrated its trafficking from the apical endfoot to the nucleus upon cleavage. Our results identified the apical endfoot as the central site of active Notch signaling to securely prohibit inappropriate differentiation of neural progenitors.

本文言語English
ページ(範囲)1671-1682
ページ数12
ジャーナルDevelopment (Cambridge)
141
8
DOI
出版ステータスPublished - 2014 4

ASJC Scopus subject areas

  • 分子生物学
  • 発生生物学

フィンガープリント

「Cadherin-based adhesions in the apical endfoot are required for active Notch signaling to control neurogenesis in vertebrates」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル