c-Kit immunoexpression delineates a putative endothelial progenitor cell population in developing human lungs

Takaya Suzuki, Satoshi Suzuki, Naoya Fujino, Chiharu Ota, Mitsuhiro Yamada, Takashi Suzuki, Mutsuo Yamaya, Takashi Kondo, Hiroshi Kubo

研究成果: Article査読

24 被引用数 (Scopus)

抄録

Expression of c-Kit and its ligand, stem cell factor (SCF), in developing human lung tissue was investigated by immunohistochemistry. Twenty-eight human fetal lungs [age range 13 to 38 gestational wk (GW)] and 12 postnatal lungs (age range 1-79 yr) were evaluated. We identified c-Kit+ cells in the lung mesenchyme as early as 13 GW. These mesenchymal c-Kit+ cells in the lung did not express mast cell tryptase or α-smooth muscle actin. However, these cells did express CD34, VEGFR2, and Tie-2, indicating their endothelial lineage. Three-dimensional reconstructions of confocal laser scanning images revealed that c-Kit+ cells displayed a closed-end tube formation that did not contain hematopoietic cells. From the pseudoglandular phase to the canalicular phase, c-Kit+ cells appeared to continuously proliferate, to connect with central pulmonary vessels, and finally, to develop the lung capillary plexus. The spatial distribution of c-Kit- and SCF-positive cells was also demonstrated, and these cells were shown to be in close association. Our results suggest that c-Kit expression in early fetal lungs marks a progenitor population that is restricted to endothelial lineage. This study also suggests the potential involvement of c-Kit signaling in lung vascular development.

本文言語English
ページ(範囲)L855-L865
ジャーナルAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
306
9
DOI
出版ステータスPublished - 2014 5 1

ASJC Scopus subject areas

  • 生理学
  • 呼吸器内科
  • 生理学(医学)
  • 細胞生物学

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