Bipartite functional map of the E. coli RNA polymerase α subunit: Involvement of the C-terminal region in transcription activation by cAMP-CRP

Kazuhiko Igarashi, Akira Ishihama

研究成果: Article査読

256 被引用数 (Scopus)

抄録

The α subunit of Escherichia coli RNA polymerase plays a major role in the subunit assembly. Carboxy-terminal deletion derivatives lacking 73 or 94 amino acid residues were assembled in vitro into enzyme molecules. Core enzymes consisting of these C-terminal-truncated α subunits were as active in RNA synthesis as native core enzyme. By the addition of σ70 subunit, these mutant enzymes initiated transcription from certain promoters. The mutant RNA polymerases, however, did not show cAMP-CRP activated transcription. These results demonstrate that the N-terminal region of the α subunit is involved in the formation of active enzyme molecule, while the C-terminal region plays an essential role in response to transcription activation by cAMP-CRP.

本文言語English
ページ(範囲)1015-1022
ページ数8
ジャーナルCell
65
6
DOI
出版ステータスPublished - 1991 6 14
外部発表はい

ASJC Scopus subject areas

  • 生化学、遺伝学、分子生物学(全般)

フィンガープリント

「Bipartite functional map of the E. coli RNA polymerase α subunit: Involvement of the C-terminal region in transcription activation by cAMP-CRP」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル